rabbit anti d2r extracellular antibody  (Alomone Labs)

Journal: ACS Chemical Neuroscience

Article Title: Multicolor Quantum Dot Tracking Uncovers Phenotypic Rescue of DAT A559V Aberrant Diffusion Upon D2R Antagonism

doi: 10.1021/acschemneuro.5c00897

Figure Lengend Snippet: Cartoon model for DAT-D2S membrane microdomain coconfinement. The outward-facing conformation of DAT and DAT A559V transiently enters D2S membrane microdomains without forming a direct physical interaction with D2S, in which DAT N-terminal phosphorylation levels regulate the duration of microdomain coconfinement.

Article Snippet: Rabbit anti-D2R (extracellular) antibody (ADR-002) was purchased from Alomone Laboratories.

Techniques: Membrane, Phospho-proteomics

Journal: PLOS ONE

Article Title: Characterization of the atypical antipsychotic drug aripiprazole cytotoxicity in the neutrophil model cell line HL-60

doi: 10.1371/journal.pone.0318878

Figure Lengend Snippet: Dopamine receptor subtype agonists and antagonists used in this study.

Article Snippet: The following primary antibodies were used to detect specific dopamine receptor subtypes: PE mouse anti-human Dopamine D1 receptor (BioLegend), rabbit anti-dopamine D2R (extracellular) receptor (Alomone labs), rabbit anti-dopamine D3R receptor (MilliporeSigma), rabbit anti-dopamine D4R receptor (Invitrogen), and rabbit anti-dopamine D5R receptor (Invitrogen).

Techniques:

Journal: Cerebral Cortex (New York, NY)

Article Title: Dopamine Receptor Expression Among Local and Visual Cortex-Projecting Frontal Eye Field Neurons

doi: 10.1093/cercor/bhz078

Figure Lengend Snippet: Antibody information

Article Snippet: DAPI stains DNA and is therefore a label for all nucleated cells. table ft1 table-wrap mode="anchored" t5 Table 3 caption a7 Antigen Host Vendor information Dilution D1R Rabbit polyclonal ADR-001; Alomone Labs, Jerusalem, Israel 1:200 D2R Goat polyclonal SC-7522; Santa Cruz Biotechnology, Santa Cruz, CA 1:200 D2R Rabbit polyclonal ADR-002; Alomone Labs, Jerusalem, Israel 1:200 Neurogranin Mouse monoclonal SC-514922; Santa Cruz Biotechnology, Santa Cruz, CA 1:200 SMI-32 Mouse monoclonal NE1023; Millipore, Temecula, CA 1:1000 Parvalbumin Mouse monoclonal P3088; Sigma-Aldrich Inc., St. Lous, MO 1:1000 Calbindin Mouse monoclonal CB300; Swant Inc., Switzerland 1:1000 Calretinin Mouse monoclonal 6B3; Swant Inc., Switzerland 1:1000 Somatostatin Rat monoclonal MAB354; Millipore, Temecula, CA 1:400 Open in a separate window Antibody information

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: A2A-D2 Heteromers on Striatal Astrocytes: Biochemical and Biophysical Evidence

doi: 10.3390/ijms20102457

Figure Lengend Snippet: A2A-D2 heterodimers on rat striatal astrocytes: proximity ligation assay. Detection of in situ PLA A2A-D2 heteroreceptor complexes was carried out with primary antibodies (rabbit polyclonal anti-A2AR, mouse monoclonal anti-D2R and goat polyclonal anti-GFAP) in rat striatal slices. ( A ) The merge of the maximum intensity projections of a representative field (240x240 µm; z 10 µm) is shown; GFAP (red), DAPI (blue), PLA for A2A-D2 heteroreceptor complexes appears as yellow clusters. The boxed region (i) is shown at a higher magnification in ( B ). ( C ) Merged confocal images of a single z stack of the boxed region (ii) at a higher magnification: double immunolabeling for GFAP (red) and PLA A2A-D2 heteroreceptor complexes (green). ( D ) Colocalized map of the boxed region (ii); the colocalization analysis plugins—colocalization threshold with GFAP as ROI—was used to create the colocalized map; ImageJ Fiji software. A complete lack of stain for PLA A2A-D2 heteroreceptor complexes was obtained in the negative control experiments, performed avoiding the conjugation of a primary antibody with the Duolink Probes. In the figure the merges of the maximum intensity projections of two representative fields are shown: PLA for A2A-D2. heteroreceptor complexes without the primary anti-A2A antibody ( E ) or the primary anti-D2 antibody ( F ). Scale bar 25 µm or 10 µm

Article Snippet: In situ PLA was performed on 10μm rat striatal slices using the primary antibodies (mouse anti-A2A (1:200, Merck Millipore Corporation, 05-717, Burlington, MA, USA); rabbit anti-D2R (1:200, Alomone Labs, ADR-002, Jerusalem, Israel); goat anti-GFAP (1:500, Santa Cruz Biotechnoloy Inc, Dallas, TX, USA, sc-6170), the Duolink in situ PLA detection kit (DUO92014, Sigma-Aldrich, St Louis, MO, USA) and Alexa Fluor 546-conjugated donkey anti-goat (1:500; Molecular Probes, Eugene, OR, USA).

Techniques: Proximity Ligation Assay, In Situ, Confocal, Immunolabeling, Analysis, Software, Staining, Negative Control, Conjugation Assay